Lonza Nucleofector 2b單孔細(xì)胞核轉(zhuǎn)染系統(tǒng)
——原代細(xì)胞、難轉(zhuǎn)染細(xì)胞系高效轉(zhuǎn)染解決方案
北京澤平代理的德國(guó)進(jìn)口Lonza Nucleofector 2b單孔細(xì)胞核轉(zhuǎn)染系統(tǒng)(原Amaxa Nucleofector II/2b Device,又稱Lonza 2b細(xì)胞核轉(zhuǎn)儀、Lonza 2b細(xì)胞電轉(zhuǎn)儀), 作為全球知名的高效基因電轉(zhuǎn)儀,其利用傳統(tǒng)電穿孔原理,結(jié)合細(xì)胞特異性電轉(zhuǎn)染液,可以將包括DNA、RNA、質(zhì)粒、多肽、蛋白質(zhì)、核糖核蛋白復(fù)合體RNP、小分子化合物等轉(zhuǎn)入細(xì)胞質(zhì)中,并穿過核膜直接進(jìn)入細(xì)胞核中,實(shí)現(xiàn)高效轉(zhuǎn)染,稱為Nucleofector核轉(zhuǎn)染技術(shù)(Nucleofection)。其中質(zhì)粒DNA轉(zhuǎn)染效率最高達(dá)到90%、寡核苷酸如siRNA轉(zhuǎn)染效率最高達(dá)到99%。
Lonza 2b電轉(zhuǎn)儀自2001年上市以來,廣泛用于包括干細(xì)胞、神經(jīng)細(xì)胞、T淋巴細(xì)胞在內(nèi)的動(dòng)物原代細(xì)胞和難轉(zhuǎn)染的細(xì)胞系,以及細(xì)菌、外泌體轉(zhuǎn)染中,助力CAR-T、CAR-NK、CRISPR/Cas9基因編輯、IPS重編程、RNA干擾、外泌體遞送等多種前沿研究,全球發(fā)表文章超過14,000篇。
2b電轉(zhuǎn)儀不依賴于有絲分|裂,尤其適合不分化細(xì)胞,如靜止的T淋巴細(xì)胞、神經(jīng)細(xì)胞等,最快2小時(shí)可觀察到蛋白表達(dá)。相比脂質(zhì)體轉(zhuǎn)染、病毒轉(zhuǎn)導(dǎo)、傳統(tǒng)電穿孔儀,Lonza 2b電轉(zhuǎn)儀操作簡(jiǎn)單,重復(fù)性高,在原代細(xì)胞和細(xì)胞系中實(shí)現(xiàn)更高轉(zhuǎn)染效率、更高細(xì)胞活率、更快基因表達(dá),加快推進(jìn)實(shí)驗(yàn)進(jìn)程。
Fig. Primary NHDF-neo cells were transfected with labeled plasmid DNA encoding GFP, fixed after 2h in 3.5%PFA and analyzed by confocal microscopy.
Lonza Nucleofector核轉(zhuǎn)染技術(shù)依托電轉(zhuǎn)儀設(shè)備、電轉(zhuǎn)染試劑盒、全球共享數(shù)據(jù)庫(kù)三方面技術(shù)和資源優(yōu)勢(shì),實(shí)現(xiàn)高效、靈活、方便的細(xì)胞轉(zhuǎn)染體驗(yàn)。
①2b電轉(zhuǎn)儀——內(nèi)置針對(duì)不同細(xì)胞類型優(yōu)化的電脈沖程序,無需人工設(shè)置電壓電流等電擊參數(shù),無需摸索轉(zhuǎn)染條件,選定程序一鍵操作,實(shí)驗(yàn)過程簡(jiǎn)單方便。
②電轉(zhuǎn)染試劑盒——Lonza 2b電轉(zhuǎn)染試劑盒由電極杯(電轉(zhuǎn)杯)、電轉(zhuǎn)染緩沖液、補(bǔ)充劑、pmaxGFP陽(yáng)性對(duì)照質(zhì)粒、巴氏吸管組成。其電轉(zhuǎn)緩沖液配方根據(jù)原代細(xì)胞、細(xì)胞系類型單獨(dú)優(yōu)化,每種試劑盒用于不同細(xì)胞類型,以提高轉(zhuǎn)染效率、轉(zhuǎn)染后細(xì)胞活率,支持多種底物共轉(zhuǎn)染,實(shí)驗(yàn)結(jié)果具備高重復(fù)性。
③全球共享數(shù)據(jù)庫(kù)——線上實(shí)時(shí)數(shù)據(jù)庫(kù)(https://knowledge.lonza.com),可查詢超過759種細(xì)胞的轉(zhuǎn)染全流程數(shù)據(jù),包括轉(zhuǎn)染前細(xì)胞來源、傳代、培養(yǎng)條件、轉(zhuǎn)染程序選擇和操作技巧、轉(zhuǎn)染后培養(yǎng)條件、歷史轉(zhuǎn)染結(jié)果等,資源不斷更新,豐富便捷的數(shù)據(jù)參考,提高實(shí)驗(yàn)效率,幫助科研人員專注于研究本身。
Lonza 2b細(xì)胞電轉(zhuǎn)儀轉(zhuǎn)染操作流程
Lonza 2b電轉(zhuǎn)儀參數(shù)
品牌 |
龍沙Lonza |
產(chǎn)地 |
德國(guó)科隆 |
中文名稱 |
Nucleofector 2b單孔細(xì)胞核轉(zhuǎn)染系統(tǒng) |
英文名稱 |
Nucleofector II/2b Device |
型號(hào) |
II/2b |
貨號(hào) |
AAB-1001 |
分類 |
基因電穿孔轉(zhuǎn)染儀器 |
用途 |
懸浮細(xì)胞、貼壁細(xì)胞消化后轉(zhuǎn)染 |
通量 |
單個(gè)樣本 |
反應(yīng)體系 |
100μL |
細(xì)胞數(shù)量 |
105至107 |
適用細(xì)胞 |
①動(dòng)物原代細(xì)胞、細(xì)胞系,物種包括各類哺乳動(dòng)物、雞、斑馬魚、果蠅等 |
②原核微生物細(xì)菌 |
③外泌體等 |
轉(zhuǎn)染底物 |
DNA、RNA(mRNA、miRNA、siRNA等)、質(zhì)粒、多肽、蛋白質(zhì)、核糖核蛋白復(fù)合體RNP、小分子化合物 |
尺寸 |
30×23×11cm |
重量 |
2.8kg |
電源 |
240V-110V,50-60Hz,自我調(diào)節(jié) |
銷售授權(quán) |
中國(guó)大陸一級(jí)代理商(不含港澳臺(tái)) |
貨期 |
大量現(xiàn)貨 |
服務(wù) |
售前技術(shù)咨詢,裝機(jī),售后服務(wù)(售后限北京澤平銷售儀器) |
引用文獻(xiàn)
1. Korbinian N. Kropp, et al.(2023) Targeting the melanoma-associated antigen CSPG4 with HLA-C*07:01-restricted T-cell receptors. https://doi.org/10.3389/fimmu.2023.1245559
2. Stephan Riesenberg, et al.(2023) Efficient high-precision homology-directed repair-dependent genome editing by HDRobust. https://doi.org/10.1038/s41592-023-01949-1
3. Kui Zhao, et al.(2023) Generation of an NSD2-deficient human embryonic stem cell line using CRISPR/Cas9 technology. https://doi.org/10.1016/j.scr.2023.103255
4. Meiling Jiang, et al.(2023) Generation of a homozygous RANGRF knockout hiPSC line by CRISPR/Cas9 system. https://doi.org/10.1016/j.scr.2023.103136
5. Xingjie Ren, et al.(2023) Efficient bi-allelic tagging in human induced pluripotent stem cells using CRISPR. https://doi.org/10.1016/j.xpro.2023.102084
6. Ittetsu Nakajima, et al.(2023) In Vivo Delivery of Therapeutic Molecules by Transplantation of Genome-Edited Induced Pluripotent Stem Cells. https://doi.org/10.1177/09636897231173734
7. Shidong Qiu, et al.(2023) Generation of the induced pluripotent stem cell line SFMUi001-A from a patient with usher syndrome type 2 caused by biallelic variants in the USH2A gene. https://doi.org/10.1016/j.scr.2023.103101
8. Chiami Moyama, et al.(2023) Myb Repression Mediates Stat5b-knockdown-induced Apoptosis and Inhibits Proliferation of Glioblastoma Stem Cells. https://doi.org/10.21873/cgp.20374
9. Saito, M.K., et al.(2023) A disease-specific iPS cell resource for studying rare and intractable diseases. https://doi.org/10.1186/s41232-023-00294-2
10. Joseph T. Smith, et al.(2023) Developmental dynamics of mitochondrial mRNA abundance and editing reveal roles for temperature and the differentiation-repressive kinase RDK1 in cytochrome oxidase subunit II mRNA editing. https://doi.org/10.1128/mbio.01854-23
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——Lonza中國(guó)一級(jí)代理商,北京澤平
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